Composite
Part:BBa_K1813025
Designed by: UBC iGEM 2015 Group: iGEM15_British_Columbia (2015-08-05)
CYP6G1 Expression Cassette with LacI Reversed
A construct used to achieve the bacterial expression of CYP6G1 (BBa_K1813004) under the regulative authority of the LacI repressor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2348
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2357
Background
Repressed by the presence of the LacI regulatory element, this construct does not allow significant expression of CYP6G1 without induction by the presence of Isopropyl β-D-1-thiogalactopyranoside in a bacterial host.
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Categories
Parameters
None |